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G-17 Quantitative Test Kit
● Instrumentation:
MKF-101 fluorescent immunoanalyzer
MKF-201 Portable dry fluorescence immunoassay analyzer
MKF-202 fluorescent immunoanalyzer
● Detection time: 5 minutes
● Storage: Room temperature (4-30°C)
● Shelf life: 24 months
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INTENDED USE
The G-17 Quantitative Test Kit along with the Medomic reader is a fluorescence immunoassay that quantifies the total Gastrin-17 (G-17) concentration in whole blood, plasma, and serum.
PRINCIPLE
The kit is based on the method of double antibodies sandwich immunofluorescence. Mix the detection buffer and sample, so that the fluorescence-labeled gastrin 17 detector antibody in buffer solution combines with gastrin 17 antigen in sample to form antigen-antibody complexes.
The complexes are captured to another gastrin 17 antibody that has been immobilized on test cassette as sample mixture migrates nitrocelluose matrix. Thus the more gastrin 17 antigen in the sample, the more complexes accumulated on the test line. Signal intensity of fluorescence on detector antibody reflects the amount of antigen captured. The concentration of gastrin 17 in sample can be detected by Medomics Fluorescent Immunoassay Reader. Medomics Reader. displays the result in terms of pmol/L.
MAIN COMPONENTS
The kit contains the corresponding number of test cassettes for each package size: the test cassette consists of a test strip shell and a test strip, which consists of a binding pad (containing fluorescent microsphere-labelled anti-G-17 specific antigen monoclonal antibody I and chicken IgY), a nitrocellulose membrane (with a C-wire encapsulated quality control antibody and
another anti-G-17 antigen monoclonal antibody II), a sample pad, blotting paper, and a PVC plate.
Dilution solution: composed of phosphate, preservative, etc., pH=7.0~7.8
APPLICABLE INSTUMENT
1.MKF-101 fluorescent immunoanalyzer
2.MKF-201 Portable dry fluorescence immunoassay analyzer
3.MKF-202 fluorescent immunoanalyzer
SAMPLE REQUIREMENTS
This product requires the collection of human whole blood, serum or plasma samples for testing.
1.Whole blood collection: blood is collected using an anticoagulant tube, and the collected blood sample is added and shaken well and set aside. The sample should not be frozen and should be tested as soon as possible after collection. If the test cannot be completed within 12 hours, it should be stored at 2°C to 8°C for no more than 2 days.
2.Serum or plasma collection: Serum and plasma should be separated as soon as possible after blood collection to avoid haemolysis. Separated serum and plasma should be tested as soon as possible, if the test can not be completed within 12 hours, placed in 2 ℃ ~ 8 ℃ storage, not more than 7 days. Long-term storage should be placed in -20 ℃ frozen, not more than 6 months.
Samples should not be frozen or thawed more than 2 times.
Samples must be returned to room temperature before testing. Frozen samples must be completely thawed, mixed well, and returned to room temperature before use, and repeated freezing and thawing should be avoided.
关键词:
test kit
ivd
rapid test kit
G-17
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E-mail: overseas@medomics-dx.com
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