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PGI/PG II Quantitative Test Kit
● Instrumentation:
MKF-101 fluorescent immunoanalyzer
MKF-201 Portable dry fluorescence immunoassay analyzer
MKF-202 fluorescent immunoanalyzer
● Detection time: 5 minutes
● Storage: Room temperature (4-30°C)
● Shelf life: 24 months
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INTENDED USE
The PGI/PG II Quantitative Test Kit along with the Medomic reader is a fluorescence immunoassay that quantifies the total Pepsinogen I (PG I) and thetotal Pepsinogen II (PG II) concentration in human serum.
INTRODUCTION
Pepsinogen is inactive precursor of pepsin in gastric juice, and divided into PG I and PG II. PGI is primary produced by fundic gland cells and mucous neck cells. PG II is produced by fundic gland cells, mucous neck cell, pyloric gland and duodenum. PG I is the index for the function of gastric secretary gland cells. PG II is associated with gastric mucosal lesion. The ratio of PG I and PG II is associated with gastric mucosal atrophy.
PRINCIPLE
The kit is based on the method of double antibodies sandwich immunofluorescence. Mix the detection buffer and sample, so that the fluorescence-labeled PG I or PG II detector antibody in buffer solution combines with PG I or PGII antigen in sample to form antigen-antibody complexes or Mix the detection buffer and sample, The complexes are captured to another PG I or PGII antibody that has been immobilized on test cassette as sample mixture migrates nitrocelluose matrix. Thus the more PG I or PGII antigen in the sample, the more complexes accumulated on the test line. Signal intensity of fluorescence on detector antibody reflects the amount of antigen captured. The concentration of PG I or PGII in sample can be detected by Medomics Fluorescent
Immunoassay Reader. Medomics Reader. displays the result in terms of ng/mL.
MAIN COMPONENTS
The kit contains the corresponding number of test cassettes for each package specification: the test cassette consists of a test strip shell and a test strip, which consists of a binding pad (containing fluorescent microsphere-labelled monoclonal antibodies against PGI and PGII antigens and chicken IgY), a nitrocellulose membrane (another set of monoclonal antibodies against PGI antigens, another set of monoclonal antibodies against PGII antigens and a C-wire wrapped QC antibody), a sample pad, an absorbent paper and PVC plate.
Dilution solution: composed of phosphate, preservative, etc., pH=7.2~7.6
APPLICABLE INSTUMENT
1.MKF-101 fluorescent immunoanalyzer
2.MKF-201 Portable dry fluorescence immunoassay analyzer
3.MKF-202 fluorescent immunoanalyzer
SAMPLE REQUIREMENTS
This product requires the collection of human whole blood, serum or plasma samples for testing.
Serum collection: Serum and plasma should be separated as soon as possible after blood collection to avoid haemolysis. Separated serum should be tested as soon as possible, if the test can not be completed within 12 hours, placed in 2℃ ~ 8℃ storage, not more than 7 days.
Long-term storage should be placed in -20℃ frozen, not more than 6 months. Samples should not be frozen or thawed more than 2 times.
Samples must be returned to room temperature before testing. Frozen samples must be completely thawed, mixed well, and returned to room temperature before use, and repeated freezing and thawing should be avoided.
关键词:
test kits
ivd
rapid test kit
PGI
PG II
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