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Dengue Ns1 & lgM/lgG Test Kit
● Instrumentation: not required
● Detection time: 15-20 minutes
● Storage: Stored away from direct sunlight at 4℃ to 30℃
● Shelf life: 24 months
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0.0
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浏览量:
1000
产品编号
所属分类
Dengue
数量
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库存:
0
1
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FOR PROFESSIONAL USE ONLY
Product Name
Dengue NS1 Ag/IgG/IgM Combo Rapid Test
Intended Use
The Dengue NS1 Ag/IgG/IgM Combo Test is a rapid chromatographic immunoassay reagent for the qualitative detection of dengue IgG and IgM antibodies and NS1 antigen in serum, plasma and whole blood to aid in the diagnosis of dengue viral infection.
Test Principle
Dengue NS1
In this test procedure, anti-Dengue NS1 antibody 2 is coated in the test line region (T). After a serum or plasma sample is placed in the sample well, it reacts with anti-Dengue NS1 antibody 1 labeled particles that have been applied to the colloidal gold conjugate pad. This mixture moves chromatographically along the length of the test strip and interacts with the coated anti-Dengue NS1 antibody 2. If the sample contains dengue virus NS1 antigen, a colored line will appear in the test line region (T) indicates a positive result. If the sample does not contain dengue virus NS1 antigen, a colored line will not appear in the test line region (T) indicates a negative result.
To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of sample has been added and membrane wicking has occurred.
Dengue IgG/IgM
When the sample contains the Dengue IgG or/and IgM antibody, it forms a complex with the colloidal gold labeled dengue recombinant antigen. The complex moves forward under the action of chromatography and combines with the mouse anti-human IgG or/and IgM antibody which coated at the T1 (IgG) or/and T2 (IgM) line to form a colored line (T1 or/and T2 line), indicates a
positive result. When the sample does not contain the Dengue IgG or/ and IgM antibody, no complex can be formed at the T1 or/and T2 line, and no colored line appears, which indicates a negative result.
Regardless of whether the Dengue IgM or/and IgG antibody is contained in the sample, the colloidal gold labeled Biotinylated BSA will bind with the coated streptavidin-conjugated IgG antibody at the C line to form a colored line(C line).
Main Components
Dengue NS1: Sample pad, colloidal gold conjugate pad, nitrocellulose membrane, absorbent pad, PVC board. Colloidal gold conjugate pad labeled with anti-Dengue NS1 antibody 1, nitrocellulose membrane coated with anti-Dengue NS1 antibody 2, control line coated with goat anti-mouse IgG antibody.
Dengue IgG/IgM: Sample pad, colloidal gold conjugate pad, nitrocellulose membrane, absorbent pad, PVC board. Colloidal gold conjugate pad labeled with dengue recombinant antigen and Biotinylated BSA, nitrocellulose membrane coated with mouse anti-human IgG antibody (T1) and mouse anti-human IgM antibody (T2), control line coated with streptavidin-conjugated IgG.
Sample diluent: Phosphate containing buffer.
Disposable plastic straws (optional).
Description: Different components of different batches cannot be used at the same time to avoid erroneous results.
Storage And Expiry
Store in a dry environment at 4-30°C, avoid hot and sunshine, valid for 24 months. DO NOT FREEZE. Some protective measures should be taken in hot summer and cold winter to avoid high temperature or freeze-thaw.
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